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Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. Our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities.<\/jats:p>","DOI":"10.1101\/2020.05.21.109546","type":"posted-content","created":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T05:55:12Z","timestamp":1590213312000},"source":"Crossref","is-referenced-by-count":23,"title":["A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation"],"prefix":"10.64898","author":[{"given":"Antonio E.","family":"Muruato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila R.","family":"Fontes-Garfias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano A.","family":"Garcia-Blanco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vineet D.","family":"Menachery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuping","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2024080406291015000_2020.05.21.109546v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"2024080406291015000_2020.05.21.109546v1.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"2024080406291015000_2020.05.21.109546v1.3","doi-asserted-by":"crossref","unstructured":"Huang, A. 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